Production of a recombinant peroxidase in different glyco-engineered Pichia pastoris strains: a morphological and physiological comparison

Table 3 Strain specific physiological parameters of the different glyco-engineered P. pastoris Mut^S strains expressing HRP C1A as model enzyme at 30 °C

	wt [12]	∆OCH1 [12]	SuperMan₅
max. µ_Gly (h⁻¹)	0.31	0.20	0.28 ± 0.03
Y_X/Gly (Cmol Cmol⁻¹)	0.41	0.54	0.55 ± 0.03
\({\text{Y}}_{{{\text{CO}}_{ 2} /{\text{Gly}}}}\) (Cmol Cmol⁻¹)	0.64	0.44	0.47 ± 0.02
Δtime_adapt (h)	19.9	8.90	4.85
q_MeOH (mmol g⁻¹ h⁻¹)	0.70	0.43	1.52 ± 0.11
Y_X/MeOH (Cmol Cmol⁻¹)	0.071	0.046	0.033 ± 0.001
\({\text{Y}}_{{{\text{CO}}_{ 2} /{\text{MeOH}}}}\) (Cmol Cmol⁻¹)	1.02	Constantly decreasing	0.97 ± 0.03
C-balance	1.09	Constantly decreasing	1.01 ± 0.05
q_p (U g⁻¹ h⁻¹)	0.77	0.50	1.05 ± 0.05

All results were gathered from a previous batch phase on glycerol, a subsequent MeOH adaptation and dynamic pulsing experiment with MeOH. Values for wt and ∆OCH1 strain were taken from [12]

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