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Table 3 Strain specific physiological parameters of the different glyco-engineered P. pastoris MutS strains expressing HRP C1A as model enzyme at 30 °C

From: Production of a recombinant peroxidase in different glyco-engineered Pichia pastoris strains: a morphological and physiological comparison

  wt [12] OCH1 [12] SuperMan5
max. µGly (h−1) 0.31 0.20 0.28 ± 0.03
YX/Gly (Cmol Cmol−1) 0.41 0.54 0.55 ± 0.03
\({\text{Y}}_{{{\text{CO}}_{ 2} /{\text{Gly}}}}\) (Cmol Cmol−1) 0.64 0.44 0.47 ± 0.02
Δtimeadapt (h) 19.9 8.90 4.85
qMeOH (mmol g−1 h−1) 0.70 0.43 1.52 ± 0.11
YX/MeOH (Cmol Cmol−1) 0.071 0.046 0.033 ± 0.001
\({\text{Y}}_{{{\text{CO}}_{ 2} /{\text{MeOH}}}}\) (Cmol Cmol−1) 1.02 Constantly decreasing 0.97 ± 0.03
C-balance 1.09 Constantly decreasing 1.01 ± 0.05
qp (U g−1 h−1) 0.77 0.50 1.05 ± 0.05
  1. All results were gathered from a previous batch phase on glycerol, a subsequent MeOH adaptation and dynamic pulsing experiment with MeOH. Values for wt and ∆OCH1 strain were taken from [12]