Skip to main content

Advertisement

Table 3 Bacterial strains and plasmids

From: Exploring the contributions of two glutamate decarboxylase isozymes in Lactobacillus brevis to acid resistance and γ-aminobutyric acid production

Strain/plasmid Characteristics Source/references
Strains
 E. coli DH5α Transformation host; F-φ80 lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rk−, mk+) phoA supE44 λ- thi-1 gyrA96 relA1 Invitrogen
 E. coli MC1061 Transformation host; λ FΔ(araA-leu)7697 araD139 Δ(codB-lac)3lac74 galK16 galE15 mcrA0 relA1 rpsL150 spoT1 mcrB9999 hsdR2 (Strr) Mobitec
 E. coli XL1-Blue Transformation host; recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac[F′ proAB lacIqZ∆M15 Tn10 Tetr] Stratagene
 L. lactis MG1363: pGhost4 L. lactis MG1363 harboring a temperature sensitive plasmid-pGhost4 [42]
 L. brevis CGMCC 1306 Wild-type strain, originally isolated from raw milk  
 L. brevis ΔgadA L. brevis CGMCC1306 derivative with gadA deleted This work
 L. brevis ΔgadB L. brevis CGMCC1306 derivative with gadB deleted This work
 L. brevis ΔgadC L. brevis CGMCC1306 derivative with gadC deleted This work
 L. brevis ΔgadAΔgadB L. brevis CGMCC1306 derivative with both gadA and gadB deleted This work
 L. brevis ΔgadB: pMG36e-gadB L. brevis ΔgadB harboring pMG36e-gadB This work
 L. brevis 9530 L. brevis CGMCC1306 harboring the NICE system helper plasmid pNZ9530 [28]
 L. brevis CK L. brevis CGMCC1306 harboring pNZ9530 and pNZ8148 [28]
 L. brevis 9530: pNZ8148-gadB L. brevis 9530 harboring pNZ8148-gadB This work
 L. brevis 9530: pNZ8148-gadCB L. brevis 9530 harboring pNZ8148-gadCB This work
 L. brevis 9530: pNZ8148-gadBC L. brevis 9530 harboring pNZ8148-gadBC This work
Plasmids
 pNZ8148 Cmr, carries the nisin-inducible promoter PnisA; 3.2 kb Mobitec
 pMG36e Emr, constitutive expression vector with P32 promoter; 3.6 kb [43]
 pMG36e-gadB Emr, gadB gene was cloned into pMG36e; 5.0 kb This work
 pNZ8148-gadCB Cmr, gadCB segment was cloned into pNZ8148; 6.1 kb This work
 pNZ8148-gadB Cmr, gadB segment was cloned into pNZ8148; 4.6 kb This work
 pNZ8148-gadBC Cmr, gadBC segment was cloned into pNZ8148; 6.1 kb This work
 pGhost4 Emr, integration vector, thermosensitive replicative plasmid in LAB, derivative of pGK12; 3.8 kb [42]