Table 3 Bacterial strains and plasmids
Strain/plasmid | Characteristics | Source/references |
|---|---|---|
Strains | ||
E. coli DH5α | Transformation host; F-φ80 lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rk−, mk+) phoA supE44 λ- thi-1 gyrA96 relA1 | Invitrogen |
E. coli MC1061 | Transformation host; λ FΔ(araA-leu)7697 araD139 Δ(codB-lac)3=Δlac74 galK16 galE15 mcrA0 relA1 rpsL150 spoT1 mcrB9999 hsdR2 (Strr) | Mobitec |
E. coli XL1-Blue | Transformation host; recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac[F′ proAB lacIqZ∆M15 Tn10 Tetr] | Stratagene |
L. lactis MG1363: pGhost4 | L. lactis MG1363 harboring a temperature sensitive plasmid-pGhost4 | [42] |
L. brevis CGMCC 1306 | Wild-type strain, originally isolated from raw milk | |
L. brevis ΔgadA | L. brevis CGMCC1306 derivative with gadA deleted | This work |
L. brevis ΔgadB | L. brevis CGMCC1306 derivative with gadB deleted | This work |
L. brevis ΔgadC | L. brevis CGMCC1306 derivative with gadC deleted | This work |
L. brevis ΔgadAΔgadB | L. brevis CGMCC1306 derivative with both gadA and gadB deleted | This work |
L. brevis ΔgadB: pMG36e-gadB | L. brevis ΔgadB harboring pMG36e-gadB | This work |
L. brevis 9530 | L. brevis CGMCC1306 harboring the NICE system helper plasmid pNZ9530 | [28] |
L. brevis CK | L. brevis CGMCC1306 harboring pNZ9530 and pNZ8148 | [28] |
L. brevis 9530: pNZ8148-gadB | L. brevis 9530 harboring pNZ8148-gadB | This work |
L. brevis 9530: pNZ8148-gadCB | L. brevis 9530 harboring pNZ8148-gadCB | This work |
L. brevis 9530: pNZ8148-gadBC | L. brevis 9530 harboring pNZ8148-gadBC | This work |
Plasmids | ||
pNZ8148 | Cmr, carries the nisin-inducible promoter PnisA; 3.2 kb | Mobitec |
pMG36e | Emr, constitutive expression vector with P32 promoter; 3.6 kb | [43] |
pMG36e-gadB | Emr, gadB gene was cloned into pMG36e; 5.0 kb | This work |
pNZ8148-gadCB | Cmr, gadCB segment was cloned into pNZ8148; 6.1 kb | This work |
pNZ8148-gadB | Cmr, gadB segment was cloned into pNZ8148; 4.6 kb | This work |
pNZ8148-gadBC | Cmr, gadBC segment was cloned into pNZ8148; 6.1 kb | This work |
pGhost4 | Emr, integration vector, thermosensitive replicative plasmid in LAB, derivative of pGK12; 3.8 kb | [42] |