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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Secretion of tumoricidal human tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) by recombinant Lactococcus lactis: optimization of in vitro synthesis conditions

Fig. 4

Optimization of hsTRAIL expression by L. lactis (hsTRAIL+) using the Nisin Controlled Gene Expression System (NICE®). TRAIL-protein was precipitated from supernatants from broth cultures of L. lactis (hsTRAIL+), induced for hsTRAIL expression with different concentrations of nisin. The samples were subjected to SDS-PAGE electrophoresis and Western blot analysis. As a negative control (NC) supernatant from non-induced broth cultures of L. lactis (hsTRAIL+) was used in parallel. For further experiments, the nisin concentration of 25 ng/ml was selected. NC—negative control; 10–80 (ng/ml)—concentration of nisin used for the induction. Data from one representative experiment out of three performed are shown

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Microbial Cell Factories

ISSN: 1475-2859

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