Strains
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CEN.PK2-1C
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Wild-type strain (MATa; his3D1; leu2-3_112; ura3-52; trp1-289; MAL2-8c; SUC2)
|
[47]
|
MG01
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CEN.PK2-1C with plasmid pDES to allow MG production
|
This work
|
MG02
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CEN.PK2-1C with plasmid pDES and plasmid pSP02 to overexpress genes from GDP-mannose pathway
|
This work
|
Plasmids
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pDES
|
pRS425 plasmid backbone with LEU2 as auxotrophic marker. Contains mgsD gene from D. mccartyi under the control of the promotor ENO2 from S. cerevisiae
|
[17]
|
pSP-GM
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pESC plasmid backbone with URA3 as auxotrophic marker. Harbors TEF1 and PGK promoters from S. cerevisiae
|
[25]
|
pSP01
|
pSP-GM with PMI40 gene from S. cerevisiae
|
This work
|
pSP02
|
pSP-GM with PSA1 and PMI40 genes from S. cerevisiae
|
This work
|