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Table 3 List of strains and plasmids used in this work

From: Production of mannosylglycerate in Saccharomyces cerevisiae by metabolic engineering and bioprocess optimization

  Relevant characteristics References
Strains
 CEN.PK2-1C Wild-type strain (MATa; his3D1; leu2-3_112; ura3-52; trp1-289; MAL2-8c; SUC2) [47]
 MG01 CEN.PK2-1C with plasmid pDES to allow MG production This work
 MG02 CEN.PK2-1C with plasmid pDES and plasmid pSP02 to overexpress genes from GDP-mannose pathway This work
Plasmids
 pDES pRS425 plasmid backbone with LEU2 as auxotrophic marker. Contains mgsD gene from D. mccartyi under the control of the promotor ENO2 from S. cerevisiae [17]
 pSP-GM pESC plasmid backbone with URA3 as auxotrophic marker. Harbors TEF1 and PGK promoters from S. cerevisiae [25]
 pSP01 pSP-GM with PMI40 gene from S. cerevisiae This work
 pSP02 pSP-GM with PSA1 and PMI40 genes from S. cerevisiae This work
  1. LEU2, leucine auxotrophy; mgsD, mannosyl-3-phosphoglycerate synthase/phosphatase gene; ENO2, gene enolase II; URA3, uracil auxotrophy; PMI40, mannose-6-phosphate isomerase gene; PSA1, GDP-mannose pyrophosphorylase gene
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Microbial Cell Factories

ISSN: 1475-2859

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