Table 3 List of strains and plasmids used in this work
| Â | Relevant characteristics | References |
|---|---|---|
Strains | ||
 CEN.PK2-1C | Wild-type strain (MATa; his3D1; leu2-3_112; ura3-52; trp1-289; MAL2-8c; SUC2) | [47] |
 MG01 | CEN.PK2-1C with plasmid pDES to allow MG production | This work |
 MG02 | CEN.PK2-1C with plasmid pDES and plasmid pSP02 to overexpress genes from GDP-mannose pathway | This work |
Plasmids | ||
 pDES | pRS425 plasmid backbone with LEU2 as auxotrophic marker. Contains mgsD gene from D. mccartyi under the control of the promotor ENO2 from S. cerevisiae | [17] |
 pSP-GM | pESC plasmid backbone with URA3 as auxotrophic marker. Harbors TEF1 and PGK promoters from S. cerevisiae | [25] |
 pSP01 | pSP-GM with PMI40 gene from S. cerevisiae | This work |
 pSP02 | pSP-GM with PSA1 and PMI40 genes from S. cerevisiae | This work |