Fig. 1

Biosynthesis of mannosylglycerate (MG) in Saccharomyces cerevisiae using glucose as carbon source. MG is produced from the reaction of GDP-mannose and 3-phosphoglycerate (3PG) with the release of GMP. To produce MG, a gene from Dehalococcoides mccartyi coding for MG synthase/phosphatase (mgsD) was cloned in a plasmid and transformed in S. cerevisiae to yield strain MG01. A second plasmid containing the genes PMI40 (encodes a mannose-6-phosphate isomerase) and PSA1 (encodes a GDP-mannose pyrophosphorylase) from S. cerevisiae was constructed and transformed in MG01 yielding MG02