Table 2 Main strains and plasmids used and developed in this study
From: Enhanced biosynthesis of arbutin by engineering shikimate pathway in Pseudomonas chlororaphis P3
Description | Source | |
|---|---|---|
Strains | ||
S17-1 (λ pir) | E. coli res− pro mod+ integrated copy of RP4, mob+, used for incorporating constructs into P. chlororaphis | Lab stock |
P. chlororaphis HT66 | P. chlororaphis wild-type, PCN, Apr, Spr | Lab stock |
P. chlororaphis P3 | A mutant from P. chlororaphis HT66 with a high PCN production | Lab stock |
HT66-4XPD | P. chlororaphis HT66, Pphz–XanB2 co-expressed to insert phzD locus | Lab stock |
HT66ΔphzE | P. chlororaphis HT66 with phzE deleted | This study |
P3ΔphzE | P. chlororaphis P3 with phzE deleted | This study |
P3ΔpobA | P. chlororaphis P3 with pobA deleted | This study |
P3-Ar0 | P3ΔpobA, MNX1 inserted to phzA locus, AS inserted to phzB locus | This study |
P3-Ar1 | P3-Ar0, XanB2 inserted to phzD locus, arbutin synthetic genes including MNX1, AS, phzC and XanB2 were expressed using Pphz | This study |
P3-Ar2 | P3-Ar1, with rpeA, lon and remE deleted | This study |
P3-Ar3 | P3-Ar2, with pykA, phzE deleted | This study |
P3-Ar3-pBBR-aroB | P3-Ar3, harboring plasmid pBBR-aroB | This study |
P3-Ar3-pBBR-aroD | P3-Ar3, harboring plasmid pBBR-aroD | This study |
P3-Ar3-pBBR-phzC | P3-Ar3, harboring plasmid pBBR-phzC | This study |
P3-Ar3-pBBR-aroD | P3-Ar3, harboring plasmid pBBR-aroD | This study |
P3-Ar3-pBBR-aroE | P3-Ar3, harboring plasmid pBBR-aroE | This study |
P3-Ar3-pBBR-ppsA | P3-Ar3, harboring plasmid pBBR-ppsA | This study |
P3-Ar3-pBBR-tktA | P3-Ar3, harboring plasmid pBBR-tktA | This study |
P3-Ar3-pBBR-XanB2 | P3-Ar3, harboring plasmid pBBR-XanB2 | This study |
P3-Ar3-pBBR-XanB2–phzC | P3-Ar3, harboring plasmid pBBR-XanB2–phzC | This study |
P3-Ar3-pBBR-MNX1–AS | P3-Ar3, harboring plasmid pBBR-MNX1–AS | This study |
P3-Ar4 | P3-Ar3, Pphz–XanB2 co-expressed to insert phzD locus | This study |
P3-Ar5 | P3-Ar4, Pphz–XanB2–phzC co-expressed to insert pykA locus | This study |
Plasmids | ||
pk18mobsacB | Broad-host-range gene replacement vector, Kmr | Lab stock |
pk18-pobA | pk18mobsacB containing pobA upstream and downstream, Kmr | This study |
pk18-lon1 | pk18mobsacB containing lon1 upstream and downstream, Kmr | This study |
pk18-lon2 | pk18mobsacB containing lon2 upstream and downstream, Kmr | This study |
pk18-rpeA | pk18mobsacB containing rpeA upstream and downstream, Kmr | This study |
pk18-remE | pk18mobsacB containing remE upstream and downstream, Kmr | This study |
pk18-pykA | pk18mobsacB containing pykA upstream and downstream, Kmr | This study |
pk18-phzE | pk18mobsacB containing phzE upstream and downstream, Kmr | This study |
pk18-XanB2 | pk18mobsacB containing XanB2, phzD upstream and downstream, Kmr | This study |
pk18-MNX1–AS | pk18mobsacB containing MNX1–AS, phzA upstream and phzB downstream, Kmr | This study |
pk18-Pphz–XanB2–phzC | pk18mobsacB containing Pphz–XanB2–phzC, pykA upstream and downstream, Kmr | This study |
pBBR1MCS | T7 expression vector, Kmr | Lab stock |
pBBR-aroB | pBBR1MCS containing aroB for overexpression, Kmr | This study |
pBBR-phzC | pBBR1MCS containing phzC for overexpression, Kmr | This study |
pBBR-aroD | pBBR1MCS containing aroD for overexpression, Kmr | This study |
pBBR-aroE | pBBR1MCS containing aroE for overexpression, Kmr | This study |
pBBR-ppsA | pBBR1MCS containing ppsA for overexpression, Kmr | This study |
pBBR-tktA | pBBR1MCS containing tktA for overexpression, Kmr | This study |
pBBR-XanB2 | pBBR1MCS containing XanB2 for overexpression, Kmr | This study |
pBBR-XanB2–phzC | pBBR1MCS containing XanB2 and phzC for overexpression, Kmr | This study |
pBBR-MNX1–AS | pBBR1MCS containing MNX1 and AS for overexpression, Kmr | This study |