Fig. 2

Effect of Acatg8 deficiency on autophagy of A. chrysogenum. a Transmission electron microscopy (TEM) analysis of WT and ∆Acatg8 under nutrition-shift condition. The autophagosomes were observed in the vacuole of WT, but not in that of ΔAcatg8 under starvation condition. V: vacuole; AB: autophagic body; M: mitochondria. Bars, 500 nm. b Distribution of autophagosomes in WT, ∆Acatg8 and Acatg8C. The autophagosomes were observed in the vacuoles of WT, but not in the vacuoles of ∆Acatg8. The complemented strain restored the wild-type phenomenon. Images were observed after 4 h induction. DIC: differential interference contrast; MDC: monodansyl cadavarine