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Fig. 5 | Microbial Cell Factories

Fig. 5

From: An oleaginous yeast platform for renewable 1-butanol synthesis based on a heterologous CoA-dependent pathway and an endogenous pathway

Fig. 5

Plasmid maps of constructs containing gene integration cassettes used in this study. The vector pYLEX-EuET carries two heterologous genes ETR1 and EutE. The vector pYLEX1-All was constructed through multiple rounds of restriction digestion and ligation for overexpressing the entire 1-butanol pathway. The vector pYLEX1-All-GPD carries all 1-butanol pathway genes and the YlGPD gene that encodes glyceraldehyde-3-phosphate dehydrogenase. The vector pYLEX1-All-MDH contains all 1-butanol pathway genes and the YlMDH gene that encodes malate dehydrogenase

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Microbial Cell Factories

ISSN: 1475-2859

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