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Table 1 Detected glycosylated and fragmented peptides of T7Muc10 using ESI–MS/MS

From: High level in vivo mucin-type glycosylation in Escherichia coli

Fragment sequence

Peptide identification probability [%]

Observed m/z

Actual peptide mass [Da]

Position of the modification within the EA2 peptide

PTTDSTTPAPT t K

97

760.87

1519.73

T12

PTTDST t PAPTTK

97

760.87

1519.73

T7

STTPAPTtKPT

100

652.83

1303.65

T12

STTPAPTTKPTtD

100

760.87

1519.73

T3

STTPAPtTKPTTD

100

760.87

1519.72

T11

  1. T7Muc10 contains six repeating EA2 peptide sequences (PTTDSTTPAPTTK). To highlight adjacent EA2 repetitions within the detected fragments, amino acids of the neighbouring EA2 amino acid sequence are underlined. Identified modified threonines carrying GalNAc residues are displayed as lower-case letters in bold type