Fig. 3From: Enhanced trypsin thermostability in Pichia pastoris through truncating the flexible regionSDS-PAGE analysis for purified WT and variants (D3, D5, D7, D9, and D11) (10 μL; protein concentration 3 μg). M, molecular weight marker; 1, WT; 2–6, D3, D5, D7, D9, and D11Back to article page