Efficient production of extracellular pullulanase in Bacillus subtilis ATCC6051 using the host strain construction and promoter optimization expression system

Table 4 Strains and plasmids

Plasmid-containing strains	Plasmids	Promoter	Host
PUL0	pBE-MCS	None	B. subtilis ATCC6051∆10
PUL1	pBEPUL01	P43	B. subtilis ATCC6051∆10
PUL2	pBEPUL02	P_spovG	B. subtilis ATCC6051∆10
PUL3	pBEPUL03	P_aprE	B. subtilis ATCC6051∆10
PUL4	pBEPUL04	P_amyE	B. subtilis ATCC6051∆10
PUL5	pBEPUL05	P_hag	B. subtilis ATCC6051∆10
PUL6	pBEPUL06	P_veg	B. subtilis ATCC6051∆10
PUL7	pBEPUL07	P_nprE	B. subtilis ATCC6051∆10
PUL8	pBEPUL08	P_nprB	B. subtilis ATCC6051∆10
PUL9	pBEPUL09	P_amyL	B. subtilis ATCC6051∆10
PUL10	pBEPUL10	P_glvA	B. subtilis ATCC6051∆10
PUL11	pBEPUL11	P_sigW	B. subtilis ATCC6051∆10
PUL201	pBEPUL201	P_spovG–P_spovG	B. subtilis ATCC6051∆10
PUL202	pBEPUL202	P_spovG–P43	B. subtilis ATCC6051∆10
PUL203	pBEPUL203	P_spovG–P_amyL	B. subtilis ATCC6051∆10
PUL204	pBEPUL204	P_spovG–P_sigW	B. subtilis ATCC6051∆10
PUL205	pBEPUL205	P_amyL–P_amyL	B. subtilis ATCC6051∆10
PUL206	pBEPUL206	P_amyL–P43	B. subtilis ATCC6051∆10
PUL207	pBEPUL207	P_amyL–P_spovG	B. subtilis ATCC6051∆10
PUL208	pBEPUL208	P_amyL–P_sigW	B. subtilis ATCC6051∆10
PUL209	pBEPUL209	P43–P43	B. subtilis ATCC6051∆10
PUL210	pBEPUL210	P43–P_spovG	B. subtilis ATCC6051∆10
PUL211	pBEPUL211	P43–P_amyL	B. subtilis ATCC6051∆10
PUL212	pBEPUL212	P43–P_sigW	B. subtilis ATCC6051∆10
PUL213	pBEPUL213	P_sigW–P_sigW	B. subtilis ATCC6051∆10
PUL214	pBEPUL214	P_sigW–P43	B. subtilis ATCC6051∆10
PUL215	pBEPUL215	P_sigW–P_spovG	B. subtilis ATCC6051∆10
PUL216	pBEPUL216	P_sigW–P_amyL	B. subtilis ATCC6051∆10

ISSN: 1475-2859