Fig. 2

Extracellular and intracellular fluorescence signals with the different secretion signals. Fluorescence signals for extracellular and intracellular E2-Crimson were measured by fluorimetry after 48 h of methanol induction using different secretion signals. Pre-pro-αf, α-factor signal sequence followed by pro-α-factor; pre-pro-αf(I), α-factor signal sequence followed by pro-α-factor(I); pre-Ost1-pro-αf, Ost1 signal sequence followed by pro-α-factor; pre-Ost1-pro-αf(I), Ost1 signal sequence followed by pro-α-factor(I). a E2-Crimson fluorescence in the culture medium for the different secretion signals. Each fluorescence signal was divided by the OD₆₀₀ at the end of the incubation. Then the signals were normalized by setting the signal for pre-pro-αf to 1. b Same as a, except that intracellular fluorescence signals were normalized by setting the signal for pre-Ost1-pro-αf(I) to 1. c Total extracellular and intracellular signals are plotted for the different secretion signals. a.u. arbitrary units