Fig. 3

Effect of gna1 on expression of the cellobiohydrolase cel7a-2 and cellulase activity under conditions involving different carbon sources. a Transcript levels of cel7a-2 genes were detected by qRT-PCR in different strains using the primers qCel7a-2 (s) and qCel7a-2 (as). All the transcripts were normalized against β-actin amplified with primers qActin (s) and qActin (as) (Additional file 1: Table S1). b Cellulase activity in different strains. Mycelia were grown in 200 ml MCC medium (C) or MCC medium supplemented with 1% glucose (G) or 1% lactose (L). After 8 days of incubation, the fermentation broth was used as the enzyme source. The statistical significance is indicated by an asterisk (P-value < 0.05 in t-test analysis) or by two asterisks (P-value < 0.01 in t-test analysis). Experiments were carried out in biological triplicates