Fig. 3
From: Evaluation of GFP reporter utility for analysis of transcriptional slippage during gene expression
The relationship between the number of preserved natural N-terminal amino-acids and fluorescence intensity of the GFP hybrids. a Details of in-frame fusion genes sequence. b Relative whole-cell fluorescence of GFP hybrids generated by T7 and E. coli RNAP. The first preserved natural N-terminal amino-acid of GFP is marked. Standard error bars from at least three determinations are shown. c Levels of the poly(A) and poly(T)-based fusion gene expressions by immunodetection of GFP