Fig. 1
From: Evaluation of GFP reporter utility for analysis of transcriptional slippage during gene expression
Effects of length of N-terminal tags on the expression and fluorescence of GFP hybrids. a, b Schematic representation and sequence details of a long (a) and short (b) tags with gfp in three reading frames (− 1, 0 and + 1). c, d Western blotting of long-tagged hybrids M2.MboIIΔ377::GFPT8 and M2.MboIIΔ414::GFPA8 detected with anti-M2.MboII (c) or anti-GFP antibodies (d), respectively. f Western blotting of short-tagged hybrids GFPA8 and GFPT8 with anti-GFP antibodies. FP full length products, SP short length products. e Relative fluorescence of the long-tagged hybrids; g relative fluorescence of the short-tagged hybrids. Standard deviations from three independent experiments are indicated