Fig. 3

Concentration (nmol/mg DCW) of metabolites involved in the GlcNAc metabolic pathway and central metabolism in S. stipitis NBRC10063 after fermenting glucose (orange bars), xylose (blue bars), or GlcNAc (red bars) for 24 h. Arrows indicate the main direction of metabolic flow. Dashed arrows indicate reactions involving more than one enzymatic step. Metabolites written in brown were not analyzed in this study. Data are presented as the means ± standard deviation (n = 3). 1,3-BPG: 1,3-bisphosphoglycerate; 2-PG: 2-phosphoglycerate; 3-PG: 3-phosphoglycerate; 6-P gluconate: 6-phosphogluconate; DHAP: dihydroxyacetone phosphate; E4P: erythrose-4-phosphate; F1,6BP: fructose-1,6-bisphosphate; F6P: fructose-6-phosphate; G3P: glyceraldehyde-3-phosphate; G6P: glucose-6-phosphate; GlcN: glucosamine; GlcN1P: glucosamine-1-phosphate; GlcN6P: glucosamine-6-phosphate; GlcNAc: N-acetyl-d-glucosamine; GlcNAc1P: N-acetyl-d-glucosamine-1-phosphate; GlcNAc6P: N-acetyl-d-glucosamine-6-phosphate; Glycerol3P: glycerol-3-phosphate; NAD⁺: nicotinamide adenine dinucleotide; NADP⁺: nicotinamide adenine dinucleotide phosphate; PEP: phosphoenolpyruvate; Ru5P: ribulose-5-phosphate; R5P: ribose-5-phosphate; S7P: sedoheptulose-7-phosphate; UDP-GlcNAc: uridine diphosphate-N-acetyl-d-glucosamine; Xu5P: xylulose-5-phosphate