Table 3 Effect of non-synonymous mutations on the expression of heterologous proteins
Strains | Activity of Est1E (U/mL) | Percentage of Est1E expression in FIM1 mutant over Est1E expression in T1 (%) | Activity of Man330 (U/mL) | Percentage of Man330 expression in FIM1 mutant over Man330 expression in T1 (%) |
|---|---|---|---|---|
T1 | 363.91 ± 12.99 | 100 | 231.29 ± 14.64 | 100 |
FIM1 sea3Δ mtc6721−903Δ ynr021wΔ | 193.81 ± 6.09 | 53.26 | 165.39 ± 6.47 | 71.5 |
FIM1 sea3Δ mtc6721−903Δ | 164.05 ± 11.45 | 45.08 | 134.07 ± 5.79 | 57.97 |
FIM1 mtc6721−903Δ | 69.95 ± 5.51 | 19.22 | 98.52 ± 11.86 | 42.6 |
FIM1 sea3Δ | 22.61 ± 3.39 | 6.21 | 83.91 ± 9.97 | 36.28 |
FIM1 ynr021wΔ | 21.57 ± 6.91 | 5.93 | 86.05 ± 10.07 | 37.2 |
FIM1 erf4Δ | 22.71 ± 5.03 | 6.24 | Non-detected | – |
FIM1 cda2Δ | 17.91 ± 1.98 | 4.92 | Non-detected | – |
FIM1 twf1Δ | 16.98 ± 0.93 | 4.67 | Non-detected | – |
FIM1 cms1Δ | 17.53 ± 4.63 | 4.82 | Non-detected | – |
FIM1 idp1Δ | 14.63 ± 2.38 | 4.02 | Non-detected | – |
FIM1 hrd3Δ | 13.35 ± 1.24 | 3.67 | Non-detected | – |
FIM1 ssk1Δ | 13.24 ± 0.47 | 3.64 | Non-detected | – |