Mutational Mtc6p attenuates autophagy and improves secretory expression of heterologous proteins in Kluyveromyces marxianus

Table 2 Non-synonymous mutations in the T1 mutant which be involved in amino-acid metabolism, protein biosynthesizing or intracellular trafficking

Genes	Mutations on ORF	Variations	Functional annotation
CMS1	A161C	Asp54 to Ala	Putative subunit of the 90S preribosome processome complex
IDP1	G1087A	Glu363 to Lys	Mitochondrial NADP-specific isocitrate dehydrogenase
CDA2	T391A	Cys131 to Ser	Chitin deacetylase, be involved in cell wall organization
HRD3	G526A	Ala176 to Thr	ER membrane protein that plays a central role in ERAD
YNR021W	1211+C	Frameshift mutation	ER membrane protein
MTC6	C755Δ	Frameshift mutation, Leu256*	Hypothetical ER and vacuolar protein
SSK1	A1505C	Asp502 to Ala	Cytoplasmic phosphorelay intermediate osmosensor and regulator
ERF4	A789T	Gln262 to His	Palmitoyltransferase subunit
SEA3	A1814T	His605 to Leu	Subunit of SEACAT
TWF1	A811G	Ile271 to Val	Highly conserved actin monomer-sequestering protein

ISSN: 1475-2859