Table 1 K. marxianus strains and mutants used in this study
Names | Genotypes or descriptions | Sources |
|---|---|---|
FIM1 | ura3Δ | This lab |
T1 | ura3Δ; efficiently secrete and express the desired proteins | This study |
FIM1 cms1Δ | ura3Δ cms1Δ | This study |
FIM1 idp1Δ | ura3Δ idp1Δ | This study |
FIM1 cda2Δ | ura3Δ cda2Δ | This study |
FIM1 hrd3Δ | ura3Δ hrd3Δ | This study |
FIM1 ynr021wΔ | ura3Δ ynr021wΔ | This study |
FIM1 mtc6721−903Δ | ura3Δ mtc6721−903Δ | This study |
FIM1 mtc6C755Δ | ura3Δ mtc6C755Δ; mimic the frameshift mutation of MTC6 in T1 mutant | This study |
FIM1 mtc6754−1773Δ | ura3Δ mtc6754−1773Δ; resulting in premature termination at Ser252 of Mtc6p | This study |
FIM1 mtc6S252* | ura3Δ mtc6C755A/T756A; resulting in premature termination at Ser252 of Mtc6p | This study |
FIM1 mtc6G59A | ura3Δ mtc6G59A; resulting in premature termination at Cys19 of Mtc6p | This study |
FIM1 ssk1Δ | ura3Δ ssk1Δ | This study |
FIM1 erf4Δ | ura3Δ erf4Δ | This study |
FIM1 sea3Δ | ura3Δ sea3Δ | This study |
FIM1 twf1Δ | ura3Δ twf1Δ | This study |
FIM1 sea3Δ mtc6721−903Δ | ura3Δ sea3Δ mtc6721−903Δ | This study |
FIM1 sea3Δ mtc6721−903Δ ynr021wΔ | ura3Δ sea3Δ mtc6721−903Δ ynr021wΔ | This study |
FIM1 atg1Δ | ura3Δ atg1Δ | This study |
T1 atg1Δ | ura3Δ atg1Δ; T1 mutant as background | This study |
FIM1 mtc6C755Δ atg1Δ | ura3Δ mtc6C755Δ atg1Δ | This study |
T1 MTC6 | ura3Δ mtc6C755Δ::MTC6; T1 mutant as background | This study |
FIM1EA | ura3Δ ATG8::EGFP-ATG8 | This study |
FIM1EA mtc6C755Δ | ura3Δ ATG8::EGFP-ATG8 mtc6C755Δ | This study |
FIM1EA atg1Δ | ura3Δ ATG8::EGFP-ATG8 atg1Δ | This study |
T1EA | ura3Δ ATG8::EGFP-ATG8; T1 mutant as background | This study |
T1EA mtc6C755Δ | ura3Δ ATG8::EGFP-ATG8 mtc6C755Δ; T1 mutant as background | This study |
T1EA atg1Δ | ura3Δ ATG8::EGFP-ATG8 atg1Δ; T1 mutant as background | This study |