Fig. 5

Evaluation of the relationship between Mtc6p and autophagy. a Alignment of the nucleotide sequences and deduced amino acid sequences between the wild-type and the mutational MTC6; PTC was abbreviated from premature termination codon; b enzymatic activity of Est1E expressed and secreted by the indicated recombinants with/without specific mutations on the ORF of MTC6 or deletion of ATG1; c cumulant of the vacuolar proc.EGFP fragments in the indicated lysates, α-tubulin was designed as internal standard; d quantitatively analysis for relative cumulant of the proc.EGFP fragments according to its gray intensity of bands showed in c; bars ± SD; **p < 0.01 vs FIM1/Est1E recombinant, ^##p < 0.01 vs T1/Est1E recombinant; NS no statistical significance