Fig. 2

Transcriptomic analysis and experimental verification. a Relative transcriptional profile of genes engaged in the intracellular vesicle trafficking, oxidative stress response and autophagy pathways; b proliferation and resistance of the FIM1/Est1E or the T1/Est1E recombinants against BFA treatment; c Western blot analysis to monitor the cumulant of the vacuolar proc.EGFP fragments, α-tubulin was designed as internal standard; d quantitatively analysis for relative cumulant of the proc.EGFP fragments according to its gray intensity of bands showed in c, the cumulant of proc.EGFP fragments was normalized with the intensity of its respective α-tubulin; bars ± SD; **p < 0.01 vs FIM1/Est1E recombinant