High cell density fed-batch production of insecticidal recombinant ribotoxin hirsutellin A from Pichia pastoris

Table 1 Purification of rHtA from the fed-batch ferment supernatant

Purification steps	Total volume (ml)	rHtA (mg)	Recovery (%)
Supernatant before purification	300	20.2	100
Ni⁺ affinity chromatography	100	12.3	61
CM chromatography	4.1	6.9	34

ISSN: 1475-2859