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Fig. 3 | Microbial Cell Factories

Fig. 3

From: High cell density fed-batch production of insecticidal recombinant ribotoxin hirsutellin A from Pichia pastoris

Fig. 3

Purification of rHtA. a Purification of rHtA by Ni+-NTA chromatography. Lane 1, elution sample following loading; lane 2, elution prior to purification. b Analysis of concentrated rHtA purity following Ni+-NTA purification. Lane 23, 23-fold concentration; lane 7, sevenfold concentration; lane 8, eluted rHtA (unconcentrated); lane M, protein marker. c Elution curves of rHtA by ion-exchange chromatography. The eluents were collected according to OD280nm > 250 mAU. The blue curve and the red curve represent the UV absorbance (mAU) and ionic strength (mS/cm) of the eluent, respectively. d SDS-PAGE analysis (15%) of rHtA by ion-exchange chromatography. Lane 1, eluted rHtA; lane 2, 23-fold concentration of the eluent from the Ni+-NTA column; lane M, protein marker. e SDS-PAGE analysis of concentrated rHtA by ion-exchange chromatography under reducing and non-reducing conditions. Lane 1, reduced conditions; lane 2, non-reducing conditions; lane M, protein marker

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Microbial Cell Factories

ISSN: 1475-2859

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