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Fig. 1 | Microbial Cell Factories

Fig. 1

From: High cell density fed-batch production of insecticidal recombinant ribotoxin hirsutellin A from Pichia pastoris

Fig. 1

Detection of rHtA expression. a SDS-PAGE analysis of secreted rHtA transformants. Lanes 1–8, BMMY culture supernatant of selected transformants from 1 g/l zeocin YPD plates; lane 9, BMMY culture supernatant of the untransformed X33 strain; lane 10, BMMY culture supernatant of empty vector transformants; lane M, protein marker. The expressed rHtA was marked with an arrow. b SDS-PAGE and western blot analysis of rHtA. Culture supernatant was collected at the indicated time (0, 1, 2, 3 and 4 days) after methanol induction in flasks. Precipitation was initiated using final concentration of 10% TCA, and proteins were analysed by 15% SDS-PAGE and stained with Coomassie Brilliant Blue R250. Lane M, protein marker

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Microbial Cell Factories

ISSN: 1475-2859

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