Table 2 Oligonucleotide primers used in this study
From: Development of a strictly regulated xylose-induced expression system in Streptomyces
Primer | Sequence |
|---|---|
XIApF | 5′-CTTCCGCGGCTCGCGGGATCTGGC |
XIpR | 5′-GCATGCATCTAGAAGCTTGAATTCGCTAGCGCTGCGGCTCCTTGCTTGCTCCG |
PLDtF | 5′-AGCGAATTCAAGCTTCTAGATGCATGCGACGACTGAGCGCCCGGACG |
PLDtR | 5′-ATTTCCGGTCGGTTCGGGGCCAGCGCAT |
PLDpF | 5′-ATATATGGTACCGGCTCCCGGGAGCTGATAGC |
PLDpR | 5′-GCATGCATCTAGAAGCTTGAATTCGCTAGCGCTGCATCCTTAAACGAAGTAAC |
PLDtR_KpnI | 5′-ATATATGGTACCATTTCCGGTCGGTTCGGGGCCAGCGCAT |
SAV5268F | 5′-ATGAGACAGCACCACAGAACGCC |
SAV5268R | 5′-ACATGCATGCCTACGCACCGGGCCAGGGAA |
XylAp_XylBp_NdeI | 5′-ATATATCATATGGCTCTCCCTCTTCCGCGGCTCGCGGGATCTG |
XylRF_NdeI | 5′-ATATATCATATGACCGCACCGCTGCACGA |
PLDtR_PstI | 5′-ATATATCTGCAGATTTCCGGTCGGTTCGGGGCCAGCGCAT |
XylRF | 5′-AATTATAGCGCTATGACCGCACCGCTGCACGA |
SG5F_BstBI | 5′-TTTTTTCGAACGCGTCGTCGTCGACGGCCT |
SG5R_BstBI | 5′-TTTTTTCGAAGATCACGAGGTCACTCCGTC |
PsXylRF_EcoRI | 5′-ATAATTGAATTCGGCTCCCGGGAGCTGATAGC |
PtXylRR_HindIII | 5′-ATGCATAAGCTTATTTCCGGTCGGTTCGGGGC |
XylRR | 5′-ATATATATGCATCTACCGGTGCGTCGCCGC |