Table 1 Plasmids and strains used in this study
From: Development of a strictly regulated xylose-induced expression system in Streptomyces
Plasmid or strain | Relevant feature |
|---|---|
Plasmid | |
 pIJ350 | Versatile vector for protein expression; thiostrepton resistance marker |
 pUC19 | Vector for cloning genes |
 pGM160 | A shuttle vector containing pSG5 ori |
 pK18mob | Cloning vector; oriT, oriV; kanamycin resistance marker |
 pXC | Vector for expression of chitobiase gene (SAV_5268) under the control of xylA promoter; thiostrepton resistance marker |
 pXCBR | Vector for expression of chitobiase gene (SAV_5268) under the control of xylA promoter and xylR gene under the control of xylB promoter; thiostrepton resistance marker |
 pLR | Vector containing pSG5 ori for expression of xylR gene under the control of pld promoter; kanamycin resistance marker |
 pXCPR | Vector for expression of chitobiase gene (SAV_5268) under the control of xylA promoter and xylR gene under the control of pld promoter; thiostrepton resistance marker |
Strain | |
 Escherichia coli strain JM109 | recA1 endA1 gyrA96 thi-1 hsdR17 (rK− mK+) e14− (mcrA−) supE44 relA1 Δ(lacproAB)/F’[traD36 proAB + lacI q lacZΔM15] |
 S. lividans 1326 | WT strain |
 XC | Streptomyces lividans strain harboring pXC vector |
 XCBR | Streptomyces lividans strain harboring pXCBR vector |
 XCr | Streptomyces lividans strain harboring pXC and pLR vectors |
 XCPR | Streptomyces lividans strain harboring pXCPR vector |