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Table 5 Experimental matrix of the 32 factorial design and results obtained of each experiment and predicted from RSM

From: Valuation of agro-industrial wastes as substrates for heterologous production of α-galactosidase

Exp no.

Coded values

Real valuesa

(U/mL)b

x 1

x 2

X 1

X 2

Observed

Estimated (Y)

1

− 1

− 1

6

2

3.813

3.84963

2

0

− 1

7.5

2

5.286

5.20958

3

1

− 1

9

2

1.945

1.98479

4

− 1

0

6

3

4.571

4.88225

5

0

0

7.5

3

5.945

5.70596

6

1

0

9

3

1.64

1.94492

7

− 1

1

6

4

5.165

4.81712

8

0

1

7.5

4

4.412

5.10458

9

1

1

9

4

1.152

0.807292

10

0

0

7.5

3

6.363

5.70596

11

0

0

7.5

3

5.324

5.70596

12

0

0

7.5

3

5.808

5.70596

  1. aX1, beet molasses (%); X2, milk whey (%)
  2. bExtracellular ScAGal activity (μmol min/mL) to pH 4 and 40 °C