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Table 3 Integration frequency and extracellular ScAGal activity of multiple copy K. lactis transformants

From: Valuation of agro-industrial wastes as substrates for heterologous production of α-galactosidase

Recombinant strain

Integration

ScAGal activitya

Type

Clones

%

U/mL

Clones

GG799MEL1His-1

Single copy

24

51

0.039 ± 0.014

3

Multi-copy

23

49

0.108 ± 0.001

18

   

0.193 ± 0.078

5

GG799MEL1His-2

Single copy

14

42

0.167 ± 0.066

3

Multi-copy

19

58

0.362 ± 0.104

11

   

0.741 ± 0.200

8

Y1140MEL1His-1

Single copy

15

42

0.005 ± 0.001

3

Multi-copy

21

58

0.010 ± 0.001

17

   

0.020 ± 0.001

4

Y1140MEL1His-2

Single copy

13

43

0.112 ± 0.030

3

Multi-copy

17

57

0.210 ± 0.029

13

   

0.372 ± 0.042

4

  1. Each 96-well dishes cultures contained three single copy transformants identified as described in Methods section, which express only one copy of ScAGal. A unit (U) is defined as μmol.min to pH 4 and 40 °C
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Microbial Cell Factories

ISSN: 1475-2859

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