Table 1 Oligonucleotides used in this study
From: Valuation of agro-industrial wastes as substrates for heterologous production of α-galactosidase
Primer | Sequence (5′ to 3′)a | Strand | Applied strategy |
|---|---|---|---|
P1 | CCGCTCGAGAAAAGAGTGTCTCCGGTTACAATGGCCTTGG | F | XhoI cleavage site |
P2 | CCCAAGCTTATGTTTGCTTTCTACTTTCTCAC | F | HindIII cleavage site |
P3 | GGAAGATCTTCAGTGGTGGTGGTGGTGGTGAGAAGAGG | R | BglII cleavage site |
P4 | GTGGTTGCTTTATTGAATGGAG | F | Sequencing |
P5 | TTCAAGTAGTCAACGCGGTTA | R | Sequencing, integration |
P6 | ACACACGTAAACGCGCTCGGT | F | Single copy integration |
P7 | CAGTGATTACATGCATATTGT | F | Multi-copy integration |
P8 | TCAAGCCTCTGTCATCGCAAT | F | MEL1 probe (qPCR) |
P9 | TCTCTTCCAAGGTCGTGTTCATT | R | MEL1 probe (qPCR) |
P10 | AAATGGAAGACAACCCGCC | F | TAF10 probe (qPCR) |
P11 | TTACGAATTTCTGTGTAGCAAGAGC | R | TAF10 probe (qPCR) |
P12 | ACACCTGTGCTGGATATCCTG | F | MEL1 probe (Southern) |
P13 | GATCATTCCAACCACCAACAC | R | MEL1 probe (Southern) |