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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Valuation of agro-industrial wastes as substrates for heterologous production of α-galactosidase

Fig. 4

Optimization of a medium based on cheese whey and beet molasses for extracellular ScAGal production by the strain KGM21 using RSM. a Pareto chart of the effects of the independent variables on the response at the 95% significance level (vertical line). b Observed versus adjusted values by the regression model. c Interaction molasses-whey effect, where the factor molasses ranges from − 1 to + 1 and the factor whey is maintained constant at the value + 1 (up line) and − 1 (down line). d Response surface-contour plot of the combined effect of molasses and whey indicating the estimated maximum value

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