Table 3 Transformation efficiency for pJP067 and organization of the specificity unit hsdsA ans hsdsB in mutants generated after transformation of pJP042
From: Genome alterations associated with improved transformation efficiency in Lactobacillus reuteri
Strain | HsdSA | HsdSB | Transformation efficiency (TE) for pJP067 | SD TE of pJP067 | ||
|---|---|---|---|---|---|---|
TRD at 5′ | TRD at 3′ | TRD at 5′ | TRD at 3′ | |||
LR 6475 | TRD1 | TRD2 | TRD3 | TRD4 | 1.33E+00 | 0.58 |
LR 4659 | TRD1 | TRD2 | TRD3 | TRD4 | 1.00E+00 | 1 |
LJO1 | TRD1 | TRD4 | TRD3 | TRD2 | 5.10E+03 | 3.27E+03 |
LJO8 | TRD1 | TRD4 | TRD3 | TRD2 | 8.47E+05 | 2.61E+05 |
LJO3 | TRD3 | TRD4 | TRD1 | TRD2 | 1.09E+06 | 5.62E+05 |
Clone B1 | TRD3 | TRD2 | TRD1 | TRD4 | 0.00E+00 | 0 |
Clone B2 | TRD1 | TRD2 | TRD3 | TRD4 | 1.50E+03 | 8.89E+02 |
Clone B3 | TRD1 | TRD4 | TRD3 | TRD2 | 2.23E+04 | 1.41E+04 |
Clone B4 | TRD1 | TRD2 | TRD3 | TRD4 | 8.33E+03 | 3.66E+03 |
Clone B5 | TRD1 | TRD2 | TRD3 | TRD4 | 0.00E+00 | 0 |
Clone S1 | TRD1 | TRD4 | TRD3 | TRD2 | 0.00E+00 | 0 |
Clone S2 | TRD3 | TRD2 | TRD1 | TRD4 | 5.00E+01 | 26.46 |
Clone S3 | TRD1 | TRD2 | TRD3 | TRD4 | 7.27E+02 | 5.36E+02 |
Clone S4 | TRD3 | TRD4 | TRD1 | TRD2 | 1.35E+05 | 8.32E+04 |
Clone S5 | TRD1 | TRD2 | TRD3 | TRD4 | 7.31E+02 | 4.19E+02 |