Table 2 Description of trgA mutations generated in LR 6475 when transformed with pSH71 derivatives

pJP042

490

C

A

Glutamine

Lysine

1283

G

A

Glycine

Aspartic acid

1506

G

C

Methionine

Isoleucine

1750

C

A

Proline

Threonine^a

1991

G

A

Glycine

Glutamic acid

2004

G

C

Methionine

Isoleucine

pSIP411

598

Deletion of 202 bp

928

C

T

Glutamine

Stop codon

1223

C

T

Serine

Leucine

1534

C

A

Glutamine

Lysine

1564

C

T

Arginine

Cysteine

1838

C

A

Serine

Stop codon

1915

C

T

Glutamine

Sop codon

2099

G

A

Tryptophan

Stop codon

pJP067

1231, 1235

G, A

A, G

Glycine, lysine

Arginine, arginine^b

Completion deletion of the gene^c

pNZ8048

80

A

G

Glutamic acid

Glycine