Table 1 List of primers used in the study

URA3_1

Selection marker

TCC ATA TGA ATT ATG CAT GCA TAA CTT CGT ATA ATG TAT GCT ATA CGA AGT TAT ACC AAA ATG CCC TCC TAC GAA GCT CGA GC

URA3_2

Selection marker

CCA CAT GTG GGA ATT CAT AAC TTC GTA TAG CAT ACA TTA TAC GAA GTT ATC GAG AAA CAC AAC AAC ATG CCC CAT TGG AC

TEF_1

TEF promoter-XPR2 terminator cassette

GGA ATT CCG GGT TTA AAC AGA GAC CGG GTT GGC GGC GTA TTT G

TEF_XPR2_1

TEF promoter-XPR2 terminator cassette

TAG GGT ACC TCT AGA CGT CCA CCC GGG AAG GAT CCT TTG AAT GAT TCT TAT ACT CAG AAG

TEF_XPR2_2

TEF promoter-XPR2 terminator cassette

CTT CTG AGT ATA AGA ATC ATT CAA AGG ATC CTT CCC GGG TGG ACG TCT AGA GGT ACC CTA

XPR2_1

TEF promoter-XPR2 terminator cassette

CCA CAT GTG GAC GTC GAC GCC ACC TAC AAG CCA GAT TTT CTA TTT AC

ARS_1

Yarrowia automatously replicating sequence

TCC ATA TGC CAG TCT ACA CTG ATT AAT TTT CGG G

ARS_2

Yarrowia automatously replicating sequence

TTG CAT GCA TAA GCT AAA AGT AAC TCG CAG CGC A

rDNA_1

Ribosomal DNA integration site

TCC ATA TGG CGG CCG CGG GTC CGG CTG CCA GTT GCC CAG CCG CCA G

rDNA_2

Ribosomal DNA integration site

ATG CAT GCT GGT GGT AGT AGC AAA TAT TCA AAT G

rDNA_3

Ribosomal DNA integration site

GCG TCG ACG TTG GCG CGC CTG CTT CGG TAT GAT AGG AAG AGC CG

rDNA_4

Ribosomal DNA integration site

CCA CAT GTG CGG CCG CGG CAG ACA CTG CGT CGC TCC GTC CAC

TEF_Casset_1

TEF promoter

GCG TCG ACA GAG ACC GGG TTG GCG GCG TAT TTG

TEF_Casset_2

TEF promoter

TTG GCG CGC CAG AGA CCG GGT TGG CGG CGT ATT TG

XPR2-Casset_1

XPR2 terminator

TTG GCG CGC CGC CAC CTA CAA GCC AGA TTT TCT ATT TAC

ACAT_F:

Acetyl-CoA thiolase

GGA TCC ATG CGA CTC ACT CTG CCC CGA CTT

ACAT_R

Acetyl-CoA thiolase

CCT AGG CTA CTC GAC AGA AGA GAC CTT CTT G

NphT7_F

Acetoacetyl CoA synthase

GGA TCC ATG ACT GAT GTC CGA TTC CGC ATT ATC

NphT7_R

Acetoacetyl CoA synthase

CCT AGG TTA CCA CTC AAT CAG AGC GAA GCT

HMGS_F

HMG-CoA synthase

GGA TCC ATG TCG CAA CCC CAG AAC GTT GG

HMGS_R

HMG-CoA synthase

CCT AGG CTA CTG CTT GAT CTC GTA CTT TCG

IPI_F

IPP isomerase

TGA TCA ATG CTT CGT TCG TTG CTC AGA GGC

IPI_R

IPP isomerase

CCT AGG TCA CGC TTC GTT GAT GTG ATG CAC

tHMGR_F

Truncated HMG-CoA reductase

TGA TCA ATG CGA GAA GTT GTG CGA ACC CAG

tHMGR-R

Truncated HMG-CoA reductase

CCT AGG CTA TGA CCG TAT GCA AAT ATT CGA AC

MK_F

Mevalonate kinase

TGA TCA ATG GAC TAC ATC ATT TCG GCG C

MK_R

Mevalonate kinase

CCT AGG CTA ATG GGT CCA GGG ACC GAT

PMK_F

Phosphomevalonate kinase

GGA TCC ATG ACC ACC TAT TCG GCT CCG GG

PMK_R

Phosphomevalonate kinase

CCT AGG CTA CTT GAA CCC CTT CTC GAG CC

MPD_F

Mevalonate pyrophosphate decarboxylase

TGA TCA ATG ATC CAC CAG GCC TCC ACC ACC

MPD_R

Mevalonate pyrophosphate decarboxylase

CCT AGG CTA CTT GCT GTT CTT CAG AGA ACC

FPPS_F

Fusion gene of farnesyl pyrophosphate synthase and geranyl pyrophosphate synthase

GGA TCC ATG CGG GAT CCA TGT CCA AGG CGA

FPPS::GGPPS_R

Fusion gene of farnesyl pyrophosphate synthase and geranyl pyrophosphate synthase

AAA TCC GCG CTG TTA TAA TCC ATA GAA CCA CCA CCC TTC TGT CGC TTG TAA ATC TTG G

FPPS::GGPPS_F

Fusion gene of farnesyl pyrophosphate synthase and geranyl pyrophosphate synthase

CCA AGA TTT ACA AGC GAC AGA AGG GTG GTG GTT CTA TGG ATT ATA ACA GCG CGG ATT T

GGPPS_R

Fusion gene of farnesyl pyrophosphate synthase and geranyl pyrophosphate synthase

C CTA GGT CAC TGC GCA TCC TCA AAG TAC TTT C

OfCCD1_F

Carotenoid cleavage dioxygenase 1

GGA TCC ATG GGT ATG CAG GGC GAG GAT GCT

OfCCD1_R

Carotenoid cleavage dioxygenase 1

CCT AGG TTA CAC CTT AGC CTG CTC CTG GAG C