Fig. 6
From: Engineering the oleaginous yeast Yarrowia lipolytica to produce the aroma compound β-ionone
Depiction of strain stability subculture experiments and results versus the number of generations accumulated. There was no selection pressure in the agar plate medium, and we estimated ~ 30 doubling times (i.e., generations) for engineered strain to form colonies from each plating based on the mass of a single cell [54]. The engineered strain was plated twice before liquid subcultures. Solid vertical line indicates transition from solid plate growths to liquid medium