Elucidating redox balance shift in Scheffersomyces stipitis’ fermentative metabolism using a modified genome-scale metabolic model

Table 2 Point-matching validation of three GEMs

Data source	Exp. cond.^a	Product	err %^b
Data source	Exp. cond.^a	Product	iSS884	iBB814	iDH814
Caspeta et al. [8]	O: 0.24 C: 3.11	EtOH	29.9	39.1	26.7
		CO₂	11.8	16	7.8
		Cell	32.1	66.1	31.8
	O: 0.35 C: 3.03	EtOH	2.3	6.6	0.29
		CO₂	22.7	28.5	21.4
		Cell	24	63.7	24
	O: 0.75 C: 2.55	EtOH	2.5	16	3.6
		CO₂	26.6	38.8	27.1
		Cell	40.7	75.7	49.9
Li [23]	O: 1.64 C: 4.10	EtOH	11.1	36.5	1.0
		CO₂	37	55	24.1
		Cell	47	30.3	24.2
	O: 6.23 C: 4.69	EtOH	0.17 ¹	2.72¹	0.8¹
		CO₂	12.2	18.9	4.3
		Cell	26.6	40.4	2.5
	O: 1.33 C: 3.28	EtOH	6.5	14.5	7.4
		CO₂	6.2	6.1	2.2
		Cell	18.2	63.2	22.2
	O: 6.00 C: 4.32	EtOH	0	2.3¹	0
		CO₂	10.5	21.3	9.7
		Cell	12.4	47	5.5

^aUnits C/O flux: mmol/(gCDW/h). In [8], xylose is the substrate. In [23], xylose is the substrate for the first two conditions and glucose for the last two conditions. Since NADPH dependent glutamate dehydrogenase (GDH3) is repressed by glucose, the corresponding reaction was disabled for the two cases where glucose is the substrate
^b\(err\% = \left| {\frac{Pred. - Exp.}{Exp.}} \right|*100\%\). When the experimental value is zero, absolute error is given (marked with ¹). Better prediction values (i.e., smaller errors) are shown in italics

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