Table 1 Kinetic parameters of KsdD3 WT and mutants toward nine substrates

47.43 ± 1.9

61.25 ± 2.1

1.29

59811 ± 823

100.00 ± 2.5

34.89 ± 2.5

114.33 ± 5.1

3.28

112588 ± 1021

188.24 ± 3.8

37.01 ± 1.8

101.24 ± 0.9

2.74

97618 ± 689

163.21 ± 3.2

42.05 ± 4.3

79.50 ± 5.4

1.89

74648 ± 1427

124.81 ± 7.6

33.25 ± 4.2

122.91 ± 3.9

3.70

118119 ± 1134

197.49 ± 4.2

38.89 ± 2.9

92.88 ± 7.1

2.39

91506 ± 1341

152.99 ± 2.7

55.35 ± 3.1

56.94 ± 5.2

1.03

49929 ± 1538

83.48 ± 4.2

44.63 ± 2.8

74.15 ± 1.8

1.66

69278 ± 838

115.83 ± 3.3

52.18 ± 3.6

58.08 ± 2.7

1.11

54469 ± 790

91.07 ± 3.3

50.04 ± 2.7

59.68 ± 4.7

1.19

56892 ± 899

95.12 ± 3.5

42.85 ± 3.3

78.05 ± 9.2

1.82

73209 ± 1382

122.40 ± 2.9

47.91 ± 2.1

60.73 ± 3.6

1.27

59446 ± 968

99.39 ± 3.4

95.08 ± 4.7

45.27 ± 3.5

0.48

31776 ± 740

53.13 ± 5.8

75.93 ± 3.8

47.32 ± 1.5

0.62

37555 ± 1185

62.79 ± 3.5

146.28 ± 5.6

39.18 ± 1.8

0.27

24294 ± 693

40.62 ± 2.3

228.26 ± 7.1

19.20 ± 4.5

0.08

11660 ± 562

19.49 ± 3.5

149.15 ± 4.6

33.59 ± 2.1

0.23

24041 ± 347

40.19 ± 6.4

171.65 ± 3.9

26.05 ± 0.7

0.15

16799 ± 197

28.09 ± 4.2

145.11 ± 4.4

40.56 ± 4.6

0.28

24385 ± 361

40.77 ± 3.2

68.49 ± 7.0

51.76 ± 3.3

0.76

41874 ± 712

70.01 ± 3.4

107.03 ± 5.1

43.11 ± 5.4

0.40

30598 ± 294

51.16 ± 2.3

57.12 ± 2.8

55.18 ± 3.7

0.97

46677 ± 649

78.04 ± 2.5

46.85 ± 0.9

62.06 ± 6.1

1.32

61375 ± 871

102.61 ± 5.2

54.91 ± 3.6

57.71 ± 8.9

1.05

52702 ± 610

88.11 ± 3.9

62.31 ± 4.8

53.47 ± 4.1

0.86

45022 ± 429

75.27 ± 2.9

40.37 ± 3.7

87.12 ± 4.2

2.16

87199 ± 192

145.79 ± 3.8

43.19 ± 2.8

74.82 ± 1.4

1.73

70217 ± 314

117.40 ± 2.2