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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Discovery and characterization of the tubercidin biosynthetic pathway from Streptomyces tubercidicus NBRC 13090

Fig. 3

Biochemical characterization of TubE as a CDG-PRPP transferase. a Schematic of the TubE-catalyzed reaction in the presence of PRPP and CDG. b HPLC analysis of the TubE reaction. TubE reaction, HPLC profile of the TubE reaction using PRPP and CDG as substrates; Negative control, HPLC profile of the reaction using PRPP and CDG as substrates but without TubE added. Compound X shows [M+H]+ ion at m/z 327.0923, with main fragment ions at 177.9827, 194.9009, 292.0454, and 309.9970, which are fully consistent with the theoretical fragmentation pattern of dephosphorylated 1

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Microbial Cell Factories

ISSN: 1475-2859

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