Fig. 5

FACS analysis of self-assembly of a Te-dCBH1, b Ct-dCelA and c Sf-dBGL1 using two scaffoldins. Cellulases were assembled on ScafCipA3, and ScafCipA3 was assembled on ScafAGA3 on the yeast cell surface. Control represents the strain without staining. The fermentation supernatant containing Te-dCBH1, Ct-dCelA and Sf-dBGL1 was concentrated about tenfold, respectively, and OD₆₀₀ of 5 of scaffoldin expressing strain was added to the concentrated enzymes for complex cellulosome assembly. Anti-DDDDK antibody were used to detect the assembly of BGL1 and CBHI, and the anti-Myc tag antibody was used to detect the assembly of CelA. Results are representative of two independent repeat experiments