Fig. 4
From: Efficient yeast surface-display of novel complex synthetic cellulosomes
Immunofluorescence micrographs and FACS analysis of each enzyme self-assembly through covalent disulfide bonds. a Control strain with expression of empty plasmids. b Assembly of Te-aCBH1 onto ScafAGA3 on yeast cell wall. c Assembly of Ct-aCelA onto ScafAGA3 on yeast cell wall. d Assembly of Sf-aBGL1 onto ScafAGA3 on yeast cell wall. Anti-DDDDK antibody was used to detect the assembly of BGL1 and CBHI, and anti-Myc tag antibody was used to detect the assembly of CelA. Control represents the strain without staining. Results are representative of two independent repeat experiments