Table 1 Strains and plasmids used in this study
From: A novel method for transforming the thermophilic bacterium Geobacillus kaustophilus
Strain and plasmid | Description | Source or references |
|---|---|---|
Strain | ||
 B. subtilis | ||
  GR138 | trpC2 pLS20cat pGR16B | [27] |
  GR23 | trpC2 amyE::(Pspank–rapLS20 spc) pLS20cat | [27] |
  PKS11 | trpC2 pLS20cat | [22] |
  PKS86 | trpC2 amyE::(Pspank–rcoLS20 spc) pLS20rco | [23] |
  STM1 | trpC2 epr::(PrpsO–dam ble) pLS20cat | This study |
  TSU077 | trpC2 epr::(PrpsO–dam ble) | This study |
  YNB051 | trpC2 amyE::(Pspank–rapLS20 spc) epr::(PrpsO–dam ble) pLS20cat pGK1 | This study |
  YNB052 | trpC2 amyE::(Pspank–rapLS20 spc) epr::(PrpsO–dam ble) pLS20cat pGK2 | This study |
  YNB059 | trpC2 amyE::(Pspank–rapLS20 spc) epr::(PrpsO–dam ble) pLS20cat | This study |
  YNB042 | trpC2 amyE::(Pspank–rapLS20 spc) pLS20cat pGK1 | This study |
  YNB032 | trpC2 epr::(PrpsO–dam ble) pLS20cat pGK1 | This study |
  YNB101 | trpC2 amyE::(Pspank–rapLS20 spc) epr::(PrpsO–dam ble) pLS20catΔoriT pGK1 | This study |
 G. kaustophilus | ||
  MK244 | ΔpyrFR ΔGK1378–GK1390 ΔGK0343–GK0346 | [17] |
  MK72 | ΔpyrFR | [18] |
Plasmid | ||
 pGK1 | pGR16B containing the replication origin of pUCG18T and kan (thermostable) | This study |
 pGK2 | pGR16B containing kan (thermostable) | This study |
 pGR16B | erm kan (thermostable) oriTLS20 | [27] |
 pLS20cat | cat | [25] |
 pLS20rco | cat rcoLS20::kan | [23] |
 pUCG18T | kan (thermostable) | [16] |
 pUC19-K7010 | ble | [33] |