Fig. 2
From: A novel method for transforming the thermophilic bacterium Geobacillus kaustophilus
Construction of plasmids pGK1 and pGK2. a The E. coli–B. subtilis shuttle vector pGR16B contains the ampicillin resistance gene (amp), the replication origin containing the Rep gene of rolling circle plasmid pTA1015 (BS ori), the replication origin of pUC19 (EC ori), the erythromycin resistance gene (erm), and oriTLS20. pGK1 was constructed from pGR16B by inserting the segment containing both the functional Geobacillus replication origin (GK ori) and the thermostable kanamycin resistance gene (kan) of pUCG18T. b pGK2 was also constructed from pGR16B by inserting only the segment containing kan of pUCG18T