Table 1 Strains and plasmids used in this study

Strains

 MG

Wild type E. coli MG1655 strain

Lab collection

 Δhns

E. coli MG1655 Δhns

This study

 H-NS(WT)

E. coli MG1655 pBR322-hns (WT), Amp^R

This study

 3-36

E. coli MG1655 pBR322-hns (3-36), Amp^R

This study

 5-30

E. coli MG1655 pBR322-hns (5-30), Amp^R

This study

 9-1

E. coli MG1655 pBR322-hns (9-1), Amp^R

This study

 9-36

E. coli MG1655 pBR322-hns (9-36), Amp^R

This study

 10-21

E. coli MG1655 pBR322-hns (10-21), Amp^R

This study

Plasmids

 pTargetF-cadA

pMB1, sgRNA-cadA, Spe^R

Gift of professor Sheng Yang

 pCas

repA101 (Ts), Pcas-cas9, ParaB-Red, lacI^q, Ptrc-sgRNA-pMB1, Kan^R

Gift of professor Sheng Yang

 pTargetT-Δhns

pMB1, sgRNA-hns, Homo X1-Homo X2 (hns), Spe^R

This study

 pBR322

pMB1, rop, Amp^R

New England Biolabs

 pBR322-hns (WT)

pMB1, rop, pNat-hns (WT)-rrnBT, Amp^R

This study

 pBR322-hns (3-36)

pMB1, rop, pNat-hns (3-36)-rrnBT, Amp^R

This study

 pBR322-hns (5-30)

pMB1, rop, pNat-hns (5-30)-rrnBT, Amp^R

This study

 pBR322-hns (9-1)

pMB1, rop, pNat-hns (9-1)-rrnBT, Amp^R

This study

 pBR322-hns (9-36)

pMB1, rop, pNat-hns (9-36)-rrnBT, Amp^R

This study

 pBR322-hns (10-21)

pMB1, rop, pNat-hns (10-21)-rrnBT, Amp^R

This study