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Table 1 Strains and plasmids used in this study

From: Engineered global regulator H-NS improves the acid tolerance of E. coli

Strains/plasmids Genotype or description Source
Strains
 MG Wild type E. coli MG1655 strain Lab collection
 Δhns E. coli MG1655 Δhns This study
 H-NS(WT) E. coli MG1655 pBR322-hns (WT), AmpR This study
 3-36 E. coli MG1655 pBR322-hns (3-36), AmpR This study
 5-30 E. coli MG1655 pBR322-hns (5-30), AmpR This study
 9-1 E. coli MG1655 pBR322-hns (9-1), AmpR This study
 9-36 E. coli MG1655 pBR322-hns (9-36), AmpR This study
 10-21 E. coli MG1655 pBR322-hns (10-21), AmpR This study
Plasmids
 pTargetF-cadA pMB1, sgRNA-cadA, SpeR Gift of professor Sheng Yang
 pCas repA101 (Ts), Pcas-cas9, ParaB-Red, lacIq, Ptrc-sgRNA-pMB1, KanR Gift of professor Sheng Yang
 pTargetT-Δhns pMB1, sgRNA-hns, Homo X1-Homo X2 (hns), SpeR This study
 pBR322 pMB1, rop, AmpR New England Biolabs
 pBR322-hns (WT) pMB1, rop, pNat-hns (WT)-rrnBT, AmpR This study
 pBR322-hns (3-36) pMB1, rop, pNat-hns (3-36)-rrnBT, AmpR This study
 pBR322-hns (5-30) pMB1, rop, pNat-hns (5-30)-rrnBT, AmpR This study
 pBR322-hns (9-1) pMB1, rop, pNat-hns (9-1)-rrnBT, AmpR This study
 pBR322-hns (9-36) pMB1, rop, pNat-hns (9-36)-rrnBT, AmpR This study
 pBR322-hns (10-21) pMB1, rop, pNat-hns (10-21)-rrnBT, AmpR This study