Metabolic engineering of Corynebacterium glutamicum for the production of cis, cis-muconic acid from lignin

Table 1 Kinetics and stoichiometry of cis, cis-muconic acid (MA) production of Corynebacterium glutamicum MA-1 from benzoic acid, catechol or phenol, and of Corynebacterium glutamicum MA-2 from catechol

Strain substrate	MA-1 benzoic acid	MA-1 catechol	MA-1 phenol	MA-1* catechol	MA-2 catechol
q_MA [mmol g⁻¹ h⁻¹]	3.3 ± 0.1	0.2 ± 0.0	0.1 ± 0.0	3.5 ± 0.1	5.2 ± 0.4
q_Glc [mmol g⁻¹ h⁻¹]	0.5 ± 0.0	0.3 ± 0.0	0.6 ± 0.0	0.4 ± 0.0	0.7 ± 0.0
Y_X/Glc [g g⁻¹]	0.46 ± 0.01	0.38 ± 0.01	0.48 ± 0.01	0.40 ± 0.02	0.40 ± 0.02
Y_MA/Aro [mol mol⁻¹]	1.00 ± 0.02	0.99 ± 0.02	0.95 ± 0.09	1.00 ± 0.01	1.00 ± 0.01

The data comprise the specific glucose uptake rate (q_Glc), the specific MA production rate (q_MA), the biomass yield (Y_X/Glc), and the MA yield (Y_MA/Aro) and represent mean values and standard deviations from three biological replicates. The culture of the MA-1* strain additionally contained benzoate as inducer

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