Presence of protein production enhancers results in significantly higher methanol-induced protein production in Pichia pastoris

Table 2 List of expression plasmid used in this study

Plasmid	Description	Short name construct	Gene of interest	Reference
pPICZαA	-/PAOX/5′UTR/MFA/TAOX; Zeo^R		–	b
pPIC9Kα	-/PAOX/5′UTR/MFA/TAOX; his4, Kan^R		–	b
pPNic384	-/PAOX/5′UTR/MFA/TAOX; his4, Kan^R		IL8	a
pPNic775	UNA1/UNA2/PAOX/UNB/MFA/TAOX; his4, Kan ^R		IL8	a
pPNic948	-/PAOX/5′UTR/MFA/TAOX; his4, Zeo^R	Ref	IL8	a
pPNic947	UNA1/UNA2/PAOX/UNB/MFA/TAOX; his4, Zeo ^R	UNall	IL8	a
pPNIc706	-/PAOX/5′UTR/MFA/TAOX; his4, Kan^R Zeo^R		IL8	a
pPNic710	-/PAOX/5′UTR/MFA/TAOX; his4, Kan^R, Zeo^R		faeA	a
pPNic957	UNA1/PAOX/5′UTR/MFA/TAOX; his4, Kan ^R , Zeo ^R	UNA1	faeA	a
pPNic958	UNA2/PAOX/5′UTR/MFA/TAOX; his4, Kan ^R , Zeo ^R	UNA2	faeA	a
pPNic959	-/PAOX/UNB/MFA/TAOX; his4, Kan^R, Zeo^R	UNB	faeA	a
pPNic964	UNA1/UNA2/PAOX/5UTR/MFA/TAOX; his4, Kan ^R , Zeo ^R	UNA1 + UNA2	faeA	a
pPNic961	UNA1/UNA2/PAOX/UNB/MFA/TAOX; his4, Kan^R, Zeo^R	UNall	faeA	a
pPNy051	UNA1/UNA2/PAOX/UNB/MFA/TAOX; his4, Kan ^R , Zeo ^R	–	–	a
pPNy063	UNA1/UNA2/PAOX/UNB/MFA/TAOX; his4, Kan ^R , Zeo ^R	UNall[EAEA]	faeA	a
pPNy060	UNA1/UNA2/PAOX/UNB/MFA/TAOX; his4, Kan ^R , Zeo ^R	–	–	a
pPNy059	UNA1/UNA2/PAOX/UNB/MFA/TAOX; his4, Kan ^R , Zeo ^R	UNall[EAYVEF]	faeA	a

References: a: This study; b: Thermo Fisher
Structural elements: PAOX1: Promoter AOX1; 5′ UTR: 5′ Untranslated Region; UNA1, UNB and UNA2: regulatory element (see text); MFA: S. cerevisiae α-mating factor pre-pro leader sequence; TAOX; terminator AOX1; his4: P. pastoris histidine selection marker; Kan^R: G418 resistance selection marker; Zeo^R: P. pastoris zeocin selection marker

ISSN: 1475-2859