Fig. 4

Differential effects of truncated Cry5Ba3Φ proteins on nematicidal activity. a, b A lower number of live nematodes in the extracts of cry5Ba3Φ-mutant Botrytis cinerea strains than that in the extracts of Botrytis cinerea transformed with pTFCM (control) (24 h, one-way ANOVA, F_6,28 = 16.633, P < 0.0001; 48 h, one-way ANOVA, F_6,28 = 10.699, P < 0.0001). c, d Differential dead:live ratio of PWNs among the six cry5Ba3Φ-mutant Botrytis cinerea strains (24 h, one-way ANOVA, F_6,28 = 22.250, P < 0.0001; d: 48 h, one-way ANOVA, F_6,28 = 20.141, P < 0.0001). Control: Botrytis cinerea (pTFCM); Mutant 1: Botrytis cinerea (pTFCM-cry5Ba3Φ aa 74–698); Mutant 2: Botrytis cinerea (pTFCM-cry5Ba3Φ aa 115–698); Mutant 3: Botrytis cinerea (pTFCM-cry5Ba3Φ aa 202–698); Mutant 4: Botrytis cinerea (pTFCM-cry5Ba3Φ aa 1–572); Mutant 5: Botrytis cinerea (pTFCM-cry5Ba3Φ aa 1–560); Mutant 6: Botrytis cinerea (pTFCM-cry5Ba3Φ aa 74–572). Different letters indicate significant differences among treatments (P < 0.05)