Fig. 9

Characteristic culture parameters of B. pumilus DSM 18097 in complete modified Poolman medium and simplified minimal medium. B. pumilus DSM 18097 was grown in complete modified Poolman medium (10 g/L glucose) defined in Table 3 as a reference and in a simplified minimal medium with cysteine, histidine, biotin and ascorbic acid identified as important nutrients. a Oxygen and carbon dioxide transfer rate (OTR, CTR), b glucose and acetate concentrations, c acetoin and butanediol concentration and d optical density as well as pH-value. Culture conditions: 250 mL shake flask, filling volume 10 mL, shaking frequency 350 rpm, shaking diameter 50 mm and temperature 37 °C