Fig. 4

Cultivation of B. pumilus DSM 18097 in modified Poolman medium containing all nucleobases/nucleosides and vitamins specified in Table 3 supplemented with different groups of amino acids. Oxygen transfer rate during cultivation of B. pumilus DSM 18097 in the complete modified Poolman medium (10 g/L glucose) as a reference and without any amino acids and in the medium containing only amino acids from a group 1, b group 2, c group 3, d group 4, e group 5. These groups are specified in Table 1. For clarity only every second measuring point over time is represented by a symbol. For most cultivations biological duplicates are shown. Culture conditions: 48-well Round Well Plate, filling volume 700 µL, shaking frequency 1100 rpm, shaking diameter 3 mm and temperature 37 °C