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Table 4 List of used primers in this work

From: Characterizing the effect of expression of an acetyl-CoA synthetase insensitive to acetylation on co-utilization of glucose and acetate in batch and continuous cultures of E. coli W

Name

Sequence (5′-3′)

acs_fw

atgAGCCAAATTCACAAACAC

acs_L641P_rev

CTTCAAGCGGCTTCTC

FS2_acs_fw

GATCGGTCTCACatgAGCCAAATTCACAAACAC

FS3_acs_rev

GATCGGTCTCAAAGCttaCGATGGCATCGCG

FS3_acs_L641P_rev

GATCGGTCTCAAAGCttaCGATGGCATCGCGATAGCCTGCTTCTCTTCAAGCGGCTTCTC

seq_fw

GCAGTCCAGTTACGCTG

seq_rev

CGTGGACCGATCATACG

acs_seq_in_fw

GCAGTATTCCGCTGAAG

acs_seq_in_rev

GGTAGCGCCTTCCAG

  1. Inserted mutations are underlined
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Microbial Cell Factories

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