Characterizing the effect of expression of an acetyl-CoA synthetase insensitive to acetylation on co-utilization of glucose and acetate in batch and continuous cultures of E. coli W

Table 1 Growth rate, specific glucose, acetate and base uptake as well as CO₂ production rates and growth rates for batch processes on glucose + acetate, glucose and acetate during exponential growth phase

	Glc + Ace VC	ACS	ACS_L641P	Glucose VC	ACS_L641P	Acetate VC	ACS_L641P
µ [h⁻¹]	0.23 ± 0.05	0.20 ± 0.03	0.27 ± 0.04	0.72 ± 0.01	0.68 ± 0.07	0.19 ± 0.03	0.18 ± 0.04
q_GLC [mmol g⁻¹ h⁻¹]	2.85 ± 0.29	2.71 ± 0.81	3.20 ± 0.38	7.24 ± 0.18	6.90 ± 0.89	–	–
q_ACE [mmol g⁻¹ h⁻¹]	1.76 ± 0.26	1.91 ± 0.58	4.72 ± 0.26	–	–	12.42 ± 2.13	12.36 ± 1.96
q_CO2 [mmol g⁻¹ h⁻¹]	8.23 ± 1.96	5.96 ± 2.26	16.33 ± 0.88	16.16 ± 0.11	23.98 ± 0.78	12.79 ± 0.31	17.03 ± 3.32
q_NH3 [mmol g⁻¹ h⁻¹]	3.55 ± 0.58	3.29 ± 0.74	1.44 ± 0.86	5.35 ± 0.97	6.30 ± 0.41	–	–

ISSN: 1475-2859