Fig. 3

Prevalence and synteny of the P. thermoglucosidasius-like [Ni-Fe] hydrogenases. a [Ni-Fe] group 1d orthologues. The ML phylogeny was determined on the basis of the trimmed alignment of nine Pha locus proteins (PhaABCDGHIJK—2206 amino acids in length). Hydrogenase genes are coloured in light blue (dark blue for large and small catalytic subunits), tatAE genes in purple and flanking genes in yellow in the synteny diagrams. b [Ni-Fe] group 2a orthologues. The ML phylogeny was determined on the basis of the trimmed alignment of 10 Phb locus proteins (PhbBCDEFHJLMN—2348 amino acids in length). Hydrogenase genes are coloured in red (dark red for large and small catalytic subunits), genes of no known function in biosynthesis and functioning of the hydrogenase in white and flanking genes in yellow in the synteny diagrams. c [Ni-Fe] group 4a orthologues. The ML phylogeny was determined on the basis of the trimmed alignment of nine Phc locus proteins (PhcABCDFGHIJ—2744 amino acids in length). Hydrogenase genes are coloured in light green (dark green for large and small catalytic subunits), anaerobic CODH genes in purple, formate dehydrogenase-related genes in blue and flanking genes in yellow in the synteny diagrams. Values on all trees reflect bootstrap analyses (n = 500 replicates) and all trees were rooted on the midpoint